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mouse anti major histocompatibility complex ii  (Bio-Rad)


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    Bio-Rad mouse anti major histocompatibility complex ii
    Mouse Anti Major Histocompatibility Complex Ii, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti major histocompatibility complex ii/product/Bio-Rad
    Average 93 stars, based on 144 article reviews
    mouse anti major histocompatibility complex ii - by Bioz Stars, 2026-03
    93/100 stars

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    In vitro biological effects of PFP-OLNDs in BV2 microglia. BV2 microglia were pre-incubated with different concentrations of PFP-OLNDs for 3 hours and then exposed to LPS for 24 hours. (A, B) ROS formation detected by flow cytometry. (C) Representative histograms of CD45, MHC class II and CD86 expression on microglia of different groups. Unstained control (Con) is indicated in the shaded histogram. Blank represents the PBS-treated group. (D) Surface expression (MFI) of CD45, MHC class II and CD86 are shown. (E, F) Western blotting of IL-1β, IL-6, and iNOS. (G) ELISA of IL-1β, IL-6, and TNFα from BV2 cellular supernatant. Data are expressed as mean ± SEM ( n = 3). ** P < 0.01, *** P < 0.001, vs. LPS (one-way analysis of variance followed by Bonferroni post hoc test). DCFH-DA: 2′,7′-Dichlorodihydrofluorescein diacetate; ELISA: enzyme-linked immunosorbent assay; IL-1β: interleukin 1 beta; IL-6: interleukin-6; iNOS: inducible nitric oxide synthase; LPS: lipopolysaccharide; MFI: mean fluorescence intensity; MHC: major histocompatibility complex; PBS: phosphate buffered saline; PFP-OLNDs: perfluoropentane-based oxygen-loaded nanodroplets; ROS: reactive oxygen species; TNFα: tumor necrosis factor α.

    Journal: Neural Regeneration Research

    Article Title: Perfluoropentane-based oxygen-loaded nanodroplets reduce microglial activation through metabolic reprogramming

    doi: 10.4103/NRR.NRR-D-23-01299

    Figure Lengend Snippet: In vitro biological effects of PFP-OLNDs in BV2 microglia. BV2 microglia were pre-incubated with different concentrations of PFP-OLNDs for 3 hours and then exposed to LPS for 24 hours. (A, B) ROS formation detected by flow cytometry. (C) Representative histograms of CD45, MHC class II and CD86 expression on microglia of different groups. Unstained control (Con) is indicated in the shaded histogram. Blank represents the PBS-treated group. (D) Surface expression (MFI) of CD45, MHC class II and CD86 are shown. (E, F) Western blotting of IL-1β, IL-6, and iNOS. (G) ELISA of IL-1β, IL-6, and TNFα from BV2 cellular supernatant. Data are expressed as mean ± SEM ( n = 3). ** P < 0.01, *** P < 0.001, vs. LPS (one-way analysis of variance followed by Bonferroni post hoc test). DCFH-DA: 2′,7′-Dichlorodihydrofluorescein diacetate; ELISA: enzyme-linked immunosorbent assay; IL-1β: interleukin 1 beta; IL-6: interleukin-6; iNOS: inducible nitric oxide synthase; LPS: lipopolysaccharide; MFI: mean fluorescence intensity; MHC: major histocompatibility complex; PBS: phosphate buffered saline; PFP-OLNDs: perfluoropentane-based oxygen-loaded nanodroplets; ROS: reactive oxygen species; TNFα: tumor necrosis factor α.

    Article Snippet: Surface staining was then performed using 5 μL of the following antibodies: anti-mouse CD45-PE-Cyanine7 (eBioscience, Cat# 25-0459-42, RRID: AB_1944375), anti-mouse major histocompatibility complex (MHC) class II (IA/I-E)-PE (eBioscience, Cat# 12-5321-82, AB_465928), and anti-mouse CD86 (B7-2)-PE-Cyanine5 (eBioscience, Cat# 15-0862-82, AB_468778) for 20 minutes on ice in the dark.

    Techniques: In Vitro, Incubation, Flow Cytometry, Expressing, Control, Western Blot, Enzyme-linked Immunosorbent Assay, Fluorescence, Saline

    Histopathological features of 3 anti-MDA5-positive JIIM patients.

    Journal: Medicine

    Article Title: Clinicopathological features and treatment outcome of juvenile idiopathic inflammatory myopathies with anti-melanoma differentiation associated gene 5 antibodies: A case series study

    doi: 10.1097/MD.0000000000039523

    Figure Lengend Snippet: Histopathological features of 3 anti-MDA5-positive JIIM patients.

    Article Snippet: Serial frozen sections were stained with hematoxylin and eosin, anti-CD3 mouse monoclonal antibody (clone LN10; Zhongshan Golden Bridge Biotechnology, China), anti-CD8 rabbit monoclonal antibody (clone SP16; Zhongshan Golden Bridge Biotechnology), anti-major histocompatibility complex class-I (MHC-I) rabbit monoclonal antibody (clone EP1395Y; Abcam, UK), anti-major histocompatibility complex class-II (MHC-II) mouse monoclonal antibody (clone CR3/43; Dako, Denmark), anti-membrane attack complex (MAC) mouse monoclonal antibody (clone aE11; Dako), and anti-MxA rabbit polyclonal antibody (Abcam).

    Techniques: Expressing

    Muscle pathology of anti-MDA5-positive JIIMs. (A) Perifascicular atrophy in Patient 3 (black arrow). (B) Normal HE staining in Patient 2. (C) Perifascicular patterns of MxA expression in Patient 3 (black arrow). (D) Diffuse MHC-I expression in Patient 2. (E) MAC deposition in endomysial capillaries in Patient 3 (black arrow). (F) Few lymphocytic infiltrates in Patient 3. Scale bars = 100 μm. HE = hematoxylin and eosin; JIIM = juvenile idiopathic inflammatory myopathy; MAC = membrane attack complex; MDA5 = melanoma differentiation associated gene 5; MHC-I = major histocompatibility complex class-I; MHC-II = major histocompatibility complex class-II; MxA = myxovirus-resistance protein A.

    Journal: Medicine

    Article Title: Clinicopathological features and treatment outcome of juvenile idiopathic inflammatory myopathies with anti-melanoma differentiation associated gene 5 antibodies: A case series study

    doi: 10.1097/MD.0000000000039523

    Figure Lengend Snippet: Muscle pathology of anti-MDA5-positive JIIMs. (A) Perifascicular atrophy in Patient 3 (black arrow). (B) Normal HE staining in Patient 2. (C) Perifascicular patterns of MxA expression in Patient 3 (black arrow). (D) Diffuse MHC-I expression in Patient 2. (E) MAC deposition in endomysial capillaries in Patient 3 (black arrow). (F) Few lymphocytic infiltrates in Patient 3. Scale bars = 100 μm. HE = hematoxylin and eosin; JIIM = juvenile idiopathic inflammatory myopathy; MAC = membrane attack complex; MDA5 = melanoma differentiation associated gene 5; MHC-I = major histocompatibility complex class-I; MHC-II = major histocompatibility complex class-II; MxA = myxovirus-resistance protein A.

    Article Snippet: Serial frozen sections were stained with hematoxylin and eosin, anti-CD3 mouse monoclonal antibody (clone LN10; Zhongshan Golden Bridge Biotechnology, China), anti-CD8 rabbit monoclonal antibody (clone SP16; Zhongshan Golden Bridge Biotechnology), anti-major histocompatibility complex class-I (MHC-I) rabbit monoclonal antibody (clone EP1395Y; Abcam, UK), anti-major histocompatibility complex class-II (MHC-II) mouse monoclonal antibody (clone CR3/43; Dako, Denmark), anti-membrane attack complex (MAC) mouse monoclonal antibody (clone aE11; Dako), and anti-MxA rabbit polyclonal antibody (Abcam).

    Techniques: Staining, Expressing, Membrane